Molecular identification of Helicoverpa armigera (Noctuidae: Heliothinae) in Amambay Department, Paraguay

Marcos Arturo Ferreira Aguero; Enson Gusman Sosa; Osmar René Arias

doi:10.18004/investig.agrar.2018.junio.84-90

Authors

Marcos Arturo Ferreira Aguero Universidad Nacional de Asunción, Facultad de Ciencias Agrarias, Área de Protección Vegetal, Filial Pedro Juan Caballero. Pedro Juan Caballero, Paraguay.
Enson Gusman Sosa Universidad Nacional de Asunción, Facultad de Ciencias Agrarias, Ingeniería Agronómica, Filial Pedro Juan Caballero. Pedro Juan Caballero, Paraguay.
Osmar René Arias Universidad Nacional de Asunción, Facultad de Ciencias Agrarias. San Lorenzo, Paraguay.

DOI:

https://doi.org/10.18004/investig.agrar.2018.junio.84-90%20%20

Keywords:

H. armigera, pheromone, delta trap, molecular identification

Abstract

The Helicoverpa armigera (Noctuidae: Heliothinae) is a polyphagous and cosmopolitan pest distributed throughout South America. The objective of this research was to identify by PCR-RFLP molecular analysis, the presence of H. armigera in the Amambay region in Paraguay. Delta traps with sexual pheromone were deployed in the 2016/2017 season soybean crop distributed at the following locations: Pedro Juan Caballero, Zanja Pytã, Capitán Bado, Karapã’ĩ and Bella Vista Norte. Sampling cloth was used for collecting caterpillars. The collected specimens were sorted by external morphological characters, individualized in labeled jars containing pure alcohol. 24 samples were sent to the Arthropods Molecular Ecology Laboratory ESALQ/USP for identification by PCR-RFLP molecular analysis. Ten specimens of H. armigera belonging to Fortuna and Zanja Pytã were identified. Ten other specimens were identified as H. zea belonging to Cerro Cora'i, Captain Bado, Cerro Kuatiá and Karapã’ĩ. The remaining four samples did not amplify by PCR-RFLP. The analysis revealed the occurrence of H. armigera in soybean and maize crops in the northern region of Paraguay.

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